FIG. 5.

Nuclear recruitment of a pUL69-NLS mutant by heterodimerization with pUL69 homologs. HeLa cells were either transfected solely with a plasmid for FLAG-tagged pUL69 harboring point mutations within its nuclear localization signal (Flag-UL69mutNLS; a to d) or in combination with Myc-tagged pUL69 (e to h) or one of the pUL69 homologs (i to C). Two days later, indirect immunofluorescence analyses were performed using primary antibodies RAb-FLAG and MAb-Myc and anti-rabbit FITC or anti-mouse Cy3 as a secondary antibody. For visualization of cell nuclei, costaining of cells with DAPI was carried out.