TABLE 1.
Primers used in this study
| Primer | Sequence (5′ to 3′) |
|---|---|
| 5CCMVC69nestedO | GCCGCGCTTTCCGCGTGCCC |
| 5CCMVC69nestedI | ATATATTAGCAGGCCGCGTG |
| 5CCMV69EcoRV | GCTAGATATCCATGGAGCTGCACGGGCGTG |
| 5RhCMV69BamH1 | CAGTGGATCCTCATTCGAACGCGAAGAACGTGC |
| 5MCMV69EcoRV | ATCGGATATCCATGCTGCGGACCGGTGTCAAGAGACG |
| 5HHV6U42bamH1 | CAGTGGATCCTATCCTCGTGGAGTAAAACGCTC |
| 5ELHVU42nestedO | CAGCTAGATGATCTGTCCCC |
| 5ELHVU42nestedI | GGGAACAGCAACGCCAGCAG |
| 5ELHVU42Bam H1 | GCATGGATCCATGGAATCTGGAAGGCGG |
| 3CCMVC69nestedO | GAACATCCGAATCGGGGTGG |
| 3CCMVC69nestedI | GGGAGAGAGACAGGCAAGTC |
| 3CCMV69XhoI | CAGTCTCGAGTCAGTACTCGTCCATGTCGTCGCTG |
| 3RhCMV69XhoI | CAGTCTCGAGCTACAAATATCCCTCTTCATCGTC |
| 3MCMV69XbaI | ATCGTCTAGACTAGTCAGTCAGAGTCCATCTCGCTGTAGG |
| 3HHV6U42XhoI | CAGTCTCGAGTTATTCTGAGTCAGAAGAACATG |
| 3ELHVU42nestedO | CATAGTATAATGATCCGAGG |
| 3ELHVU42nestedI | CGCTACACATTATAAATTGC |
| 3ELHVU42EcoRI | GCATGAATTCCTACAGCGTCATATCGCTC |
| C69mutUAPc | CCTCAGCGAGCGAGAAGCCGCCGCGGCCGCGGCACGGCGCTTCTG |
| C69mutUAPnc | CAGAAGCGCCGTGCCGCGGCCGCGGCGGCTTCTCGCTCGCTGAGG |
| Rh69mutUAPc | GCGACCGAGAACGCCGTGCTCGGCGGGCACGGCGCTTCTGC |
| Rh69mutUAPnc | GCAGAAGCGCCGTGCCCGCCGAGCACGGCGTTCTCGGTCGC |
| 5GalKUL69as2forw | GAGCTGCACTCACGCGGCCGTCACGACGCTCCGTCGTTATCTT |
| CCCTGAGCCTGTTGACAATTAATCATCGGCA | |
| 3GalKUL69as360rev | GTTGACGGGTAGCCCGTGCAGGTGGTGGTACTTCATGTAGCT |
| GAGCGTTTCTCAGCACTGTCCTGCTCCTT | |
| 5UL69aa1 | ATGGAGCTGCACTCACGCGGCCGTC |
| 3UL69aa367 | CTCCAGATAGGGATCGTGCGGGTTG |
| n-termofUL69 | CAACGCCAAAAACGTCCAC |
| c-termofUL69 | CACCAAGCTCAGGCACGC |
| 3UL69aa440Eco | GCATGAATTCGGCCATGGCGCGGTGCATGG |