FIG. 6.

Recombinant VIG displays a high specific binding activity toward VACV antigens. The reactivity of purified recombinant VIG (orange circles) against VACV particles (Lister and IHD-J) and recombinant antigens (A27L, A33R, B5R, and VCP) was evaluated by ELISA. Commercial, plasma-derived VIG (gray diamonds) and a negative-control polyclonal antibody (anti-rhesus D [57]; black squares) were similarly tested for comparison. All determinations were performed in duplicate, and the results are presented as means ± standard errors of the means.