FIG. 2.

Granules containing PABP and poly(A)⁺ RNA but not Rox8-GFP or Rin-GFP are formed in CrPV-infected cells. S2 cells transiently transfected with expression vectors containing Rox8-GFP (A), Rin-GFP (B), GFP-PABP (C), FMR1-V5 (D), or V5-eIF3 S9 (F) were mock infected or infected for 2, 4, or 6 h with CrPV at an MOI of 10. The V5 fusion proteins and O-GlcNAc-modified proteins (E) were detected by staining using anti-V5 and anti-GlcNAc antibodies, respectively. (G) Quantitation of the percentage of cells containing poly(A)⁺ granules. (H) Western blot analysis of the indicated SG protein markers in mock- and CrPV-infected cells at 6 hpi. (I) Inhibition of SGs in Drosophila C virus-infected cells. S2 cells transiently transfected with the expression vector containing Rox8-GFP were mock-infected or infected for 12 h with DCV at an MOI of 10. Poly(A)⁺ RNA was detected by in situ hybridization using Cy3-oligo(dT) (40) (red fluorescence). Percentages represent the proportion of transfected cells containing at least three granules expressing GFP or staining with V5 or O-GlcNAc. Enlarged views of the boxed areas highlight different channels and merged views. More than 100 cells were analyzed in multiple fields for each experiment. The average of three independent experiments is shown.