FIG. 7.

Virus-induced poly(A)⁺ RNA granules do not contain CrPV RNA. S2 cells were mock or CrPV infected (MOI of 10) for 2, 4, or 6 h and then processed for in situ hybridization. Poly(A)⁺ RNA was detected by in situ hybridization using a Cy5- or Cy3-oligo(dT) (40) (red). Viral dsRNA intermediates were detected by indirect immunofluorescence using an anti-dsRNA antibody (green). CrPV RNA was detected by using a Cy5-conjugated antisense probe that hybridized to the sense strand of CrPV RNA (green). Enlarged views of boxed areas highlight different channels and merged views.