FIG. 2.

Inhibition of Nxf1 production and function by RNAi. (A) Non targeting control (C) or anti-Nxf1 (Nxf1) interfering RNAs were introduced into HeLa cells as described in Materials and Methods. After the periods indicated, cells were harvested and the concentrations of Nxf1 and β-actin in total cell extracts examined by immunoblotting. (B) Plasmids carrying a CAT reporter gene lacking (CTE−) or containing (CTE+) the MPMV constitutive transport element and control or anti-Nxf1 interfering RNAs were introduced into HeLa cells as described in Materials and Methods. At 24 h after introduction of interfering RNAs, CAT activity was assayed in cytoplasmic extracts by measurement of synthesis of tritiated n-butyryl chloramphenicol. All samples were assayed in duplicate, and the cpm values corrected for background (b) in reactions that contained no cytoplasmic cell extracts. Error bars indicate average deviations.