TABLE 1.

Dominant-negative Nxf1 inhibits production of protein V in Ad5-infected cells

Expt	Exogenous FLAG-Nxf1a	No. of cellsb		% FLAG-Nxf1-producing cells that synthesize V
		FLAG positive	FLAG and V positive
1	WT	44	40	90.9
	DN	44	3	6.8
2	WT	86	56	65.1
	DN	44	8	18.1
3	WT	136	67	49.3
	DN	86	8	9.3

^aWT, wild type; DN, dominant negative.

^bThe numbers of cells exhibiting nuclear FLAG-Nxf1 signals in individual fields such as those shown in Fig. 4A (experiment 1) and in two additional experiments were counted by using the cell-counting function of Image J (73). This same function was then used to determine the numbers of such cells that also exhibited nuclear protein V staining.