FIG. 5.

Comparison of the growth kinetics of reassortant viruses in CEFs and DEFs and polymerase activity in 293T cells. CEFs (A) or DEFs (B) were inoculated with rgCkYM7, rgCkYM-PB2^Dk/NP^Dk, rgCkYM-NP^Dk, or rgCkYM-PB2^Dk at an MOI of 0.001, and the average virus titers of the culture supernatants from three wells were determined at each time point using embryonated chicken eggs. CEFs (C) or DEFs (D) were inoculated with rgDkYK10, rgDkYK-PB2^Ck/NP^Ck, rgDkYK-NP^Ck, or rgDkYK-PB2^Ck at an MOI of 0.001, and the average virus titers were determined as described above.