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Analysis of IE1 expression in HCMV.Ap1-infected cells. HEL299 fibroblasts were mock infected or infected at an MOI of 0.6 (for the 6- and 12-h-p.i. time points) or 0.1 (for the 24-, 48-, and 72-h-p.i. time points) with HCMV, HCMV.Ap1a, and HCMV.Ap1b. When indicated, cells were treated with TPA. At designated times p.i., cells were lysed and subjected to Western blot analysis with an HCMV IE1-specific antibody. Anti-β-actin antibody was used as an internal control.
