FIG. 4.

Multiple markers of SGs partially colocalize in granules, and granules contain poly(A) RNA. (A) U2OS (i and ii) or HeLa (iii) cells were infected with ΔE3L mutant virus at an MOI of 5. Cells were fixed at 7 hpi and costained with antibodies to the SG/granule components TIA-1 (i, green) and G3BP (i, red) or eIF3b (ii, green) and G3BP (ii, red) and with DAPI to stain DNA. (B) U2OS cells were infected with ΔE3L mutant (i to iv and vi) or WT (vii and viii) VV at an MOI of 5 or mock infected (v). At 7 hpi, cells were fixed and processed for FISH using a fluorescein-tagged oligo(dT) probe to localize mRNA. As a control, a sample infected with ΔE3L mutant virus was incubated without probe (vi). Maximum-intensity projections are shown. Boxed areas show zoomed regions.