FIG. 5.

Granule formation appears to inhibit further SG formation, and granules are stable following treatment with CHX. U2OS cells were infected with ΔE3L mutant virus at an MOI of 1 (A, C) or mock infected (B). Some samples were treated with 0.1 mM NA at 5 hpi and fixed at 7 hpi (A). Further samples were treated with 0.1 mM NA at 5 hpi for 1 h and then treated with 100 μg/ml CHX for 1 h and fixed at 7 hpi (B, C). Postfixation, cells were stained for G3BP (red) and with DAPI (blue) to stain DNA. Maximum-intensity projections are shown.