FIG. 3.

GLUT1 expression is inhibited by IE72. (A) GLUT1 mRNA levels decreased in HFs transiently transfected with HCMV IE72. HFs were electroporated with pRSV72, pRSV86, pCD-MIE, or a control vector (see Materials and Methods). At 48 h after electroporation, the cells were refed with serum-free DMEM. Total RNA was isolated at 72 h postelectroporation. GLUT1 and GLUT4 mRNA levels were measured by quantitative RT-PCR and normalized to PPIA mRNA levels. (B) Cultures prepared in parallel with those reported in panel A were harvested for protein level and analyzed by Western blotting for MIE proteins and actin. (C and D) Both the GLUT1 mRNA level and protein levels were decreased in ihfie1.3 cells, which stably express HCMV IE72.