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Activation of the IRF3 and NF-κB components of the IFN-β enhanceosome by infection with WT and mutant HPIV1. A549 cells were infected with WT, F170S, or P(C−) HPIV1 at an MOI of 5 for 6 or 48 h. Phosphorylated IRF3 (Ser 396), total IRF3, and total IκBβ were detected by Western blotting (WB).
