FIG. 3.

The effect of IPs on tRNA₃^Lys annealing to ShortPBS template. (A) Annealing by GagΔp6 (filled squares) and of GagΔp6 in the presence of 2 μM IP6 (open circles), 300 μM IP3 (filled triangles), and 300 μM PIP₂-diC₄ (open triangles) in 20 mM NaCl. The inset shows data for annealing by GagΔp6 in the absence and presence of 20 μM IP6 in 150 mM NaCl. (B) Annealing by CANC (left), GagΔp6-K30,32N (middle), and NC (right) in the absence (solid squares) and presence (open circles) of IP6 in 20 mM NaCl. In these annealing experiments, the ratio of tRNA₃^Lys to ShortPBS ratio is 2.5:1, and the protein concentrations are 800 nM (4.3:1, nucleotide to protein).