FIG. 4.
Recruitment and/or activation of DCs in the lymph nodes and blood after infection with rRABVs. BALB/c mice were infected i.m. with 1 × 105 FFU of different rRABVs, and draining (inguinal) lymph nodes and blood were harvested after extensive perfusion at 3, 6, and 9 dpi. Single-cell suspensions were prepared, stained with antibodies against DCs and the DC activation markers CD11c and CD86, and analyzed by flow cytometry. Representative flow cytometric plots of DCs are shown from the lymph nodes (A) and the blood (B). The results of a detailed analysis for activated DCs (CD11c+ and CD86+) at 3, 6, and 9 dpi are presented for the lymph nodes (C) and blood (D). Asterisks indicate significant differences between the indicated experimental groups (*, P < 0.05; **, P < 0.01).