TABLE 1.
Primers used for construction of recombinant RABV infectious clones
| Primer | Nucleotide sequence (5′-3′) | Sense | Enzyme |
|---|---|---|---|
| RP1 | TCCTCCGATCGTTGTCAGAAGTAAG | + | PvuI |
| RP2 | GATCTGGTTGTTAAGCGT | − | |
| RP3 | ACGCTTAACAACCAGATC | + | |
| RP4 | CTTTCCCTAGGGTTATACAGG | + | BlnI |
| RP5 | GTATAACCCTAGGAAAGGCTCCCGATTTAA | − | BlnI |
| RP6 | AACGTACGGGAGGGGTGTTAGTTTTTTTCATGGACTTG | + | BsiWI |
| GATCGTTGAAAGGACG | − | NheI | |
| RP7 | TTTTGCTAGCTTATAAAGTGCTGGGTCATCTAAGC | + | KpnI |
| RP8 | AGCCGGGTACCCGCCCTCCCTTAGCCATCCGAGT |
Bold letters (nucleotides) in sequences denote the restriction enzyme sites, and the underlined letters in the RP6 sequence indicate RABV transcription stop/start sites.