TABLE 2.
Primers used for construction of helper plasmids
| Primer | Nucleotide sequence (5′-3′) | Sense | Relative position in the genome |
|---|---|---|---|
| RPNf | GTAGCTAGCCTACAATGGATGCCGA | + | 62 |
| RPNr | TTAGGTACCTTCTTATGAGTCACTC | − | 1415 |
| RPPf | GAAGCTAGCCAAACATGAGCAAG | + | 1505 |
| RPPr | GAGAGGTACCGTTAGCAAGATG | + | 2401 |
| RPGf | GACGCTAGCAAAGATGGTTCCTCAG | − | 3309 |
| RPGr | AAAGGTACCCCAGTCCTTACAGTCT | + | 4887 |
| RPLf | GAGGCTAGCTTCAAGATGCTCGATC | − | 5404 |
| RPLr | CACAGGTACCTTAGCATGGGCAGGC | + | 11819 |
Upper primers have the BsiWI enzyme site, and lower primers have the NheI site (boldface).