FIG. 7.

Mutations in NS2 inhibit NS2 protein-protein interactions. (A) NS2(AP) was affinity captured from cell lysates at 48 h postelectroporation of Huh-7.5(BirA) cells with Jc1/NS2(AP), either with or without (WT) the indicated mutations in NS2. NS2(AP)-associated proteins were detected as in Fig. 2A. (B) Affinity purification of NS2(AP)-associated proteins with (K27A, K81A) or without (WT) mutations in NS2 and with (+NS3 Q221L, +E1 A78T) or without second-site suppressor mutations. NS2(AP)-associated proteins were detected as in Fig. 2A. This panel is a composite created from a single exposure; it is representative of additional experiments.