FIG. 3.

OTT3 functions like RBM15 in promotion of ORF59 expression. (A) ORF59 expression in HEK293 cells 24 h after cotransfection with 300 ng of ORF59 and 100 ng of ORF57, RBM15, or OTT3. Bar graphs show relative level of ORF59 RNA in each sample from Northern blot analysis after being normalized to the level of GAPDH RNA for sample loading. (B) OTT3, which functions like RBM15, preferentially promotes ORF59 RNA accumulation in the nucleus. Bar graphs below each Northern blot show relative level of ORF59 RNA in each sample after normalization to the level of the corresponding GAPDH for sample loading. U6 results indicate nuclear fractionation efficiency. The C/N ratio of ORF59 RNA in each sample set calculated from the Northern blot is shown above the bar graph.