Figure 5.

CD36 contributes to inflammation and impaired insulin signalling in macrophages with and without adipocyte co-culture. Representative immunoblots (left) and quantification (right) demonstrating CD36-dependent increased (A) phospho-c-Jun and decreased (B) phospho-AKT in RPM cultured from 4-week HFD mice for 12 h. *P < 0.05; **P < 0.01 vs. WT; n ≥ 5 per group. Representative immunoblots (left) and quantification (right) for (C) phospho-cJun and (D) phospho-tyrosine-IRS-1 from 3T3L1 adipocytes co-cultured for 24 h with 0.5 × 10⁶ RPM isolated from 8-week HFD mice of the indicated genotypes. (E) Macrophage migration to CM from WT and CD36^−/− adipocytes which had been previously exposed to oxLDL or control media as described in Supplementary material online, Methods. *P < 0.05 vs. control.