FIG. 9.

G6PD overexpression increased KCl-induced Ca²⁺ entry into CA smooth muscle. (A, B) Transfection of a DNA encoding full-length G6PD increased expression of cGFP-tagged G6PD (top) and nontagged G6PD (bottom) in CAs detected by Western blotting (A; blot is representative of four experiments) and by immunohistochemistry (B; a representative of three experiments). G6PD overexpression increased Ca²⁺ entry and CA contraction. (C) Overexpression of G6PD increased KCl (30 mM)-induced increases in global [Ca²⁺]_i measured using Ca²⁺ green (n = 5). (D) Data summarizing peak contractions of CA overexpressing G6PD normalized by peak contractions of UT controls show that CA contraction evoked by KCl (30 mM; n = 8) and U46619 (100 mM; n = 8) was increased. cGFP, coral green fluorescent protein. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article at www.liebertonline.com/ars).