Figure 4.

Reduced core body temperature in Ubb^−/− mice. (A) Mean core body temperature in wild-type and Ubb^−/− mice (+/+, n = 9; −/−, n = 6). Mean core body temperature is shown during the 6-h period as indicated by the horizontal bar. (B) Mean core body temperature in wild-type and Ubb^−/− mice (+/+, n = 4; −/−, n = 3) under constant dark (free-running) conditions. Mice were maintained under constant dark conditions for 2 weeks and mean core body temperature was measured during the last 24-h period as indicated by the horizontal bar. Ct, circadian time. (C) Mean core body temperatures in wild-type and Ubb^−/− mice (+/+, n = 9; −/−, n = 6) are shown during the food deprivation and refeeding periods. In both the wild-type and Ubb^−/− mice, core body temperatures were reduced after food deprivation and recovered during refeeding. Ubb^−/− mice consistently exhibited lower core body temperatures, reaching down to 32.5°C during the second day of food deprivation, whereas the wild-type mice maintained their core body temperature at 35°C during the same period. All data are expressed as the means ± SEM from the indicated number of 5- to 7-month-old mice. In (B), the results are average of two independent experiments. Horizontal bars indicate light and dark phases. *P < 0.05; **P < 0.01; ***P < 0.001; #P < 0.1 vs. wild-type (+/+) mice.