Table 1.

Primers used to amplify and sequence the partial BATV S, M, and L segments

Segment primer	Sequence (5′ to 3′)	Nucleotide position*	Amplicon size (nt)	Temp. (°C)
S segment primer
BATAIS1F	TGGAATTCAATGATGTCGCTGCTAAC	79–104	344	60
BATAIS1R	TATAATCAATTTTTCCGGGTCACTCACTTT	393–422	344	60
BATAIS2F	TTGGGGGCTGGAAGGTTACTGT	262–283	363	58
BATAIS2R	TATATCTTTGGCGCATGGTCTTCTCC	599–624	363	60
BATAIS3F	CTGGGCAGATGGGGAGGAG	467–485	451	56
BATAIS3R	AAACTGCAATGCTTCAAAAACAAT	894–917	451	54
M segment primer
BATAIM2F	TGTGGCCTAGCATATCACCCTTTCA	793–817	1,407	60
BATAIM2R	AGACCGGTGATGATGATCTGTAACCTCTA	2,171–2,199	1,407	60
BATAIM3F	CCTGGGGAAGCATTGTGATTACT	1,704–1,726	525	56
BATAIM3R	CTAGCCAGCGACTCTTGCCTTCC	2,206–2,228	525	60
BATAIM4F	GTCGCTGGCTAGTGCTACCTCTGG	2,217–2,240	510	60
BATAIM4R	CTGATTATTGTCGGATTTATTGGGAACCT	2,698–2,726	510	60
BATAIM5F	AAAGGTTCCCAATAAATCCGACAA	2,696–2,719	525	57
BATAIM5R	CAAATTCTTCACATCCCCAACGACTA	3,195–3,220	525	59
BATAIM6F	AGAATTTGGGTGCCTTGCTGTCA	3,213–3,235	874	59
BATAIM6R	AGATGTTTGGTCCCCTGTGCTTATTT	4,061–4,086	874	59
L segment primer
BATAIL1F	GATGGCGATTTCCTGATTAT	283–302	711	48
BATAIL1R	TGACCCCAAGAGTTTCCTATTAT	971–993	711	51

^*The nucleotide positions are given according to the numbering in the Batai virus reference strain MM2222 (GenBank accession numbers X73464, AB257763, or AY822469).