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. 2011 Jan 27;6(1):e14609. doi: 10.1371/journal.pone.0014609

Figure 2. CEACAM1 – but not other members of the CEACAM family - mediates uptake of Opa protein-negative meningococci.

Figure 2

293 cells were transfected with constructs encoding CEACAM1, CEACAM3, CEA, CEACAM6, CEACAM7, CEACAM8 or an empty control plasmid (pcDNA). Two days after transfection cells were infected for 2 h with (A) Opa protein-negative meningococci (Nm Opa-) or (B) Opa protein-positive meningococci (Nm OpaCEA) and the number of internalised bacteria was determined by gentamicin protection assays. Results represent mean ± standard deviation of three independent experiments done in triplicate. Shown is the percentage of recovered bacteria compared to CEACAM1-expressing cells.