(A, B) Growth curves of male (A) and female (B) mice fed a normal chow diet. Sixteen Rcan2
+/+, 29 Rcan2
+/−, and 15 Rcan2
−/− males were analyzed from postnatal week 3 to week 10, and 9, 16, and 7, respectively, after postnatal week 10. Eighteen Rcan2
+/+, 34 Rcan2
+/−, and 23 Rcan2
−/− females were analyzed from postnatal week 3 to week 10, and 11, 19, and 17, respectively, after postnatal week 10. **: P<0.001, ***: P<0.0001 (vs Rcan2
+/+); +: P<0.05, ++: P<0.005 (vs Rcan2
+/−). (C) Representative examples of 9-month-old Rcan2
+/+ and Rcan2
−/− males. (D) Mean body weights of ten 9-month-old Rcan2
+/+ and Rcan2
−/− males. (E) Mean tibia lengths of eight 9-month-old Rcan2
+/+ and Rcan2
−/− males. (F) Mean weight of epididymal and retroperitoneal white adipose tissue (EWAT and RWAT, respectively) in ten 9-month-old Rcan2
+/+ and Rcan2
−/− males. All values are given as mean ± s.e.m. ***: P<0.0001. Filled circles/columns indicate Rcan2
+/+ mice; open circles/columns, Rcan2
−/− mice.