Figure 7. Reduced body weights in Lep^ob/ob Rcan2 ^−/− double-mutant mice.

(A, B) Growth curves of male (A) and female (B) Lep^ob/ob and double-mutant mice fed the normal chow diet. Eleven Lep^ob/ob and 14 double-mutant males were measured from postnatal week 3 to week 10, and 7 and 9, respectively, after postnatal week 10. Eleven Lep^ob/ob and 13 double-mutant females were measured. *: P<0.05, **: P<0.01, ***: P<0.0001. (C) Appearance of 20-week-old Lep^ob/ob and double-mutant male mice. (D) Weights of epididymal and retroperitoneal white adipose tissue (EWAT and RWAT, respectively) and of liver in 6 Lep ^ob/ob and 6 double-mutant males. All values are given as mean ± s.e.m. **: P<0.005; ***: P<0.0001. Filled circles/columns indicate Lep ^ob/ob mice; open circles/columns, double-mutant mice.