Skip to main content
. 2011 Jan 27;6(1):e14599. doi: 10.1371/journal.pone.0014599

Figure 8. PKD2-induced myogenesis is mediated via Mef2D activation and Pax3 repression.

Figure 8

(A) C2C12 cells were co-transfected with Mef2D-luc or Mef2D-luc mutated, Renilla and either wild type (PKD2-WT), catalytically active mutant (PKD2-S706/710E) or catalytically inactive (PKD2-DA) PKD2 constructs, respectively. Medium change from growth to differentiation medium occurred after 36 hours. After incubation with differentiation medium for 24 hours, cells were harvested and luciferase assays were performed according to the manufacturer's instructions. Graphs were generated out of three independent and tested for significance using the Student t-test. P<0.05 (*) and p<0.01 (**) are indicated. (B) Cells were co-transfected with pcDNA3-Pax3-Flag, (PRS-9)-TK-CAT and/or pEGFP-PKD2 mutants as indicated. 24 hours upon transfection cells were harvested and CAT ELISA was performed according to the manufacturer's instructions. Graphs were generated out of three independent experiments and tested for significance using the Student t-test. P<0.001 (***).