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. 2011 Jan 27;6(1):e16416. doi: 10.1371/journal.pone.0016416

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(a) The region between the T-DNA left (LB) and right (RB) regions are shown as open rectangles. Arrows indicate orientation relative to the direction of normal transcription.Abbreviations: Nopaline synthase terminator (nos3′); Maize ubiquitin promoter and 5′ untranslated intron (Ubi+int); Hygromycin hpt antibiotic resistance marker (Hyg); Cauliflower Mosaic Virus 35S promoter (CaMV 35S); A. tumefaciens Tml 3′ transcriptional terminator (tml 3′); Barley Cre intron (Cre); Nucleotides nucleotides 153–727 of PviCAD2 (PviCAD2). A line below the drawing representing 1 kb is included for scale. The region used as a probe for DNA blot-hybridization is shown as a solid line below the drawing. (b) Reduction of coniferaldehyde (forward direction) in stem extracts. Activity is expressed as a percentage of highest level. Control untransformed regenerants and vector transformed regenerants are light orange. Individual regenerants transformed with pEC129 are shown as dark blue. Dashed light orange and dark blue lines indicate averages of the control lines and pEC129 regenerants respectively. Lines derived from ALBA22 are indicated with the prefix A22 and lines derived from ALBA4 are indicated with the prefix A4.