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. 2011 Jan 27;6(1):e16466. doi: 10.1371/journal.pone.0016466

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Chen et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Representative pictures of the cell line UD-SCC1 are shown. (A) The first generation of spheroids. (B) A subspheroid formed after seeding at a concentration of 1,000 cells/ml. (C) A spheroid formed in the 21st generation. (D) Spheroid adhering and growing to confluence after replating into flasks coated for tissue culture. (E) Parental cell line grown permanently as a monolayer culture.