Table 3. Percent dauer formation at 25°C of trx-1(ok1449); daf-28(sa191) double mutants expressing the indicated transgene.
Transgenic animals | Non-transgenic animals | ||||
Transgene | Transgenic line | % ± SEM† | N | % ± SEM† | N |
Ptrx-1::trx-1::GFP ++ | 1 | 80±1 | 284 | 39±3 | 397 |
2 | 82±3 | 245 | 48±7 | 395 | |
3 | 66±2 | 252 | 23±1 | 363 | |
Pssu-1::trx-1::GFP ++ | 1 | 74±4 | 441 | 43±14 | 471 |
2 | 78±1 | 514 | 46±3 | 496 | |
Ptrx-1::trx-1(SGPS)::GFP ++ | 1 | 81±3 | 271 | 35±6 | 305 |
2 | 86±3 | 316 | 41±1 | 385 | |
3 | 66±6 | 226 | 21±5 | 376 | |
Pgpa-4::trx-1::GFP | 1 | 53±14 | 424 | 34±9 | 531 |
2 | 36±10 | 502 | 38±9 | 472 | |
3 | 34±9 | 450 | 28±7 | 616 | |
Pges-1::trx-1::GFP | 1 | 40±12 | 518 | 37±6 | 503 |
2 | 46±13 | 501 | 39±6 | 507 | |
Ptrx-1::GFP | 1 | 43±8 | 474 | 30±12 | 470 |
2 | 48±7 | 339 | 30±10 | 479 | |
3 | 34±8 | 455 | 31±9 | 541 |
Mean percentage of dauer larvae ± standard error of the mean (SEM); 2 assays per transgenic line, except for Ptrx-1::GFP and Pgpa-4::trx-1::GFP: 3 assays. N: total (pooled) number of animals.
p<0.001 by chi-squared test.