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Interaction of DevRC (A) and full-length DevR (B), with tgs1-Rv3131 promoter DNA. Double-stranded oligonucleotides having P+S box sequences belonging to the tgs1-Rv3131 divergent promoters were incubated with increasing concentrations of DevRC or DevR. Arrow, DNA-protein complex; F, free oligonucleotides, arrowheads indicate molecular weight markers in kilobase pairs (lane M), (C) Fraction of bound DNA (from Fig. 1A, B) plotted against protein concentration.
