Table 1. Plasmids used in this study.

Plasmid	Relevant featuresa	Source/Reference
pUS-DevRC	pET28a overexpressing DevR C-terminal domain cloned in NdeI site	This study
pAV-DevR	pET28a overexpressing full length wild type DevR cloned in NdeI site	[12]
pJFR19	E. coli-Mycobacterium integrating shuttle plasmid with 3-kbacetamidase promoter, Hygr	[43]
pFPV27	E. coli-Mycobacterium shuttle plasmid with promoter less gfp, Kanr	[44]
pET28a	E. coli expression vector (with N-terminal His6 tag), Kanr	Novagen
pMG86	pJFR19 containing devR-devS expressed from acetamidase promoter, Hygr	[45]
pTGS	pFPV27 containing tgs1 promoter (-143 to +45), Kanr	[22]
p3131	pFPV27 containing Rv3131 promoter (-150 to +48), Kanr	[22]
pSD POperon devR	pJFR19 containing devR (cloned between NdeI and XbaI sites), full-length DevR is expressed from Rv3134c-devRS operon promoter (-608 to +998, ref. 20) cloned in NdeI and BstBI sites	S.D.Majumdar and J.S.Tyagi, 2010, unpublished
pUS POperon devRC	pJFR19 containing devRC (cloned between NdeI and XbaI sites), DevRC is expressed from Rv3134c-devRS operon promoter cloned in NdeI and BstBI sites	This study
pUS PAcet devRC	pJFR19 containing devRC (cloned between NdeI and XbaI sites), DevRC expressed from constitutive acetamidase promoter cloned in NdeI and BstBI sites	This study
pUS Phsp60 devRC	pJFR19 containing devRC (cloned between NdeI and XbaI sites), DevRC expressed from constitutive hsp60 promoter cloned in NdeI and BstBI sites	This study

^aThe coordinates of the promoters (in parentheses) are with reference to the transcription start point (TSP) of tgs1;

Hyg^r, hygromycin resistance;

Kan^r, kanamycin resistance.