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. 2011 Jan 27;6(1):e16500. doi: 10.1371/journal.pone.0016500

Table 3. Primers used in this study.

Primer Sequence (5′→ 3′) Application
devRC NdeI F CGGACCCATATGCAGGACCCGCTATCAGGC Cloning of devRC in pJFR19
devRC XbaI R CCGCTCTAGACCTGTTGTCATGGTCCATCACCGGGTG
hsp60 BstBI F CCGTTCGAAGGTGACCACAACGACGCGCCCGC Cloning of hsp60 promoter in pJFR19
hsp60 NdeI R CCGCATATGTGCGAAGTGATTCCTCCGGATCG
devRC NdeI F CGGACCCATATGCAGGACCCGCTATCAGGC Cloning of devRC in pET28a
devRC NdeI R CCGCATATGCTATCATGGTCCATCACCGGGTGG
RT16S F ATGACGGCCTTCGGGTTGTAA Real Time RT PCR (ref. 13)
RT16S R CGGCTGCTGGCACGTAGTTG Real Time RT PCR (ref. 13)
RT3134c F CTGGCTGGGTCGGCCTTAGC Real Time RT PCR (ref. 13)
RT3134c R TGACCTGGGAGGTTGTCG Real Time RT PCR (ref. 13)
RTdevRC F5 CGAGGATCCCTGTTGTCATGGTCCAT Real Time RT PCR
RTdevR R CGCGGCTTGCGTCCGACGTTC Real Time RT PCR
RT devS F TACTGACCGACCGGGATCGT Real Time RT PCR (ref. 13)
RTdevS R AGAGCCGCTGGATGACATGG Real Time RT PCR (ref. 13)
RT1738 F CGACGAACACGAAGGATTGA Real Time RT PCR
RT1738 R ACACCCACCAATTCCTTTTCC Real Time RT PCR
RT2031c F CGCACCGAGCAGAAGGA Real Time RT PCR
RT2031c R ACCGTGCGAACGAAGGAA Real Time RT PCR
RTtgs1 F CAGTGATTTGCGTCGCTACAG Real Time RT PCR
RTtgs1 R ACATCATTGATGGTGACGTCG Real Time RT PCR
RT3131 F CGATCAGGCCGATGTCGCCTT Real Time RT PCR
RT3131 R TCACCTCCTGGCACCGGCC Real Time RT PCR
LH1 CGAGTCGACAGAGCACGAAGGCTCGCCAGCGGAGG ACCTTTGGCCCTGCGTCGACCGA Gel shift assays (P+S box) (ref. 22)
LH2 TCGGTCGACGCAGGGCCAAAGGTCCTCCGCTGGCGA GCCTTCGTGCTCTGGTCGACTCG Gel shift assays (P+S box) (ref. 22)
3130F TGGCTGCCGGGCCTTTCCCAT DNase I footprinting (ref. 22)
3131R CATGGTCAGCGCCTTCCCCGG DNase I footprinting (ref. 22)

NdeI, XbaI, and BstBI restriction enzyme sites are underlined.