Table 3. Primers used in this study.
Primer | Sequence (5′→ 3′) | Application |
devRC NdeI F | CGGACCCATATGCAGGACCCGCTATCAGGC | Cloning of devRC in pJFR19 |
devRC XbaI R | CCGCTCTAGACCTGTTGTCATGGTCCATCACCGGGTG | |
hsp60 BstBI F | CCGTTCGAAGGTGACCACAACGACGCGCCCGC | Cloning of hsp60 promoter in pJFR19 |
hsp60 NdeI R | CCGCATATGTGCGAAGTGATTCCTCCGGATCG | |
devRC NdeI F | CGGACCCATATGCAGGACCCGCTATCAGGC | Cloning of devRC in pET28a |
devRC NdeI R | CCGCATATGCTATCATGGTCCATCACCGGGTGG | |
RT16S F | ATGACGGCCTTCGGGTTGTAA | Real Time RT PCR (ref. 13) |
RT16S R | CGGCTGCTGGCACGTAGTTG | Real Time RT PCR (ref. 13) |
RT3134c F | CTGGCTGGGTCGGCCTTAGC | Real Time RT PCR (ref. 13) |
RT3134c R | TGACCTGGGAGGTTGTCG | Real Time RT PCR (ref. 13) |
RTdevRC F5 | CGAGGATCCCTGTTGTCATGGTCCAT | Real Time RT PCR |
RTdevR R | CGCGGCTTGCGTCCGACGTTC | Real Time RT PCR |
RT devS F | TACTGACCGACCGGGATCGT | Real Time RT PCR (ref. 13) |
RTdevS R | AGAGCCGCTGGATGACATGG | Real Time RT PCR (ref. 13) |
RT1738 F | CGACGAACACGAAGGATTGA | Real Time RT PCR |
RT1738 R | ACACCCACCAATTCCTTTTCC | Real Time RT PCR |
RT2031c F | CGCACCGAGCAGAAGGA | Real Time RT PCR |
RT2031c R | ACCGTGCGAACGAAGGAA | Real Time RT PCR |
RTtgs1 F | CAGTGATTTGCGTCGCTACAG | Real Time RT PCR |
RTtgs1 R | ACATCATTGATGGTGACGTCG | Real Time RT PCR |
RT3131 F | CGATCAGGCCGATGTCGCCTT | Real Time RT PCR |
RT3131 R | TCACCTCCTGGCACCGGCC | Real Time RT PCR |
LH1 | CGAGTCGACAGAGCACGAAGGCTCGCCAGCGGAGG ACCTTTGGCCCTGCGTCGACCGA | Gel shift assays (P+S box) (ref. 22) |
LH2 | TCGGTCGACGCAGGGCCAAAGGTCCTCCGCTGGCGA GCCTTCGTGCTCTGGTCGACTCG | Gel shift assays (P+S box) (ref. 22) |
3130F | TGGCTGCCGGGCCTTTCCCAT | DNase I footprinting (ref. 22) |
3131R | CATGGTCAGCGCCTTCCCCGG | DNase I footprinting (ref. 22) |
NdeI, XbaI, and BstBI restriction enzyme sites are underlined.