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. 2011 Jan 27;6(1):e16532. doi: 10.1371/journal.pone.0016532

Figure 6. Adjuvants enhance the avidity of anti-BoNT/A β-trefoil IgG antibodies.

Figure 6

The relative avidity of anti-BoNT/A IgG serum antibodies was measured using day 162 serum using a modified ELISA as described in Materials and Methods. A. The percent antibody bound in the ELISA in the presence of 3 M ammonium thiocyanate is indicated for individual serum samples that had measurable anti-β-trefoil IgG. A higher percent antibody bound represents a greater relative avidity for vaccine-induced, antigen-specific antibody. •: represents serum anti-β-trefoil IgG with no detectable BoNT/A neutralizing activity. ○: represents serum anti-β-trefoil IgG with detectable BoNT/A neutralizing activity. B. Average percent antibody bound when samples were organized by the neutralization capacity of the serum. Vaccine-induced BoNT/A neutralizing antibody has significantly greater avidity than non-neutralizing antibody. a: % antibody bound significantly greater than in the no neutralization group. Two-tailed Mann Whitney, p = 0.0239.