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. 2011 Jan 27;6(1):e16608. doi: 10.1371/journal.pone.0016608

Figure 5. INF1 purified from P. infestans triggers cell death and a delayed ROS burst in N. benthamiana.

Figure 5

INF1[Pi] was purified from P. infestans 88069 culture supernatant and fractions loaded on a silver stained gel to confirm absence of contaminating proteins (A). Cell death inducing activity in N. benthamiana was tested by infiltration of stepwise diluted INF1[Pi] protein solution or buffer into the plant apoplast and pictures were taken 6 dpi (B). Incubation of leaf discs in INF1 or flg22-containing buffer triggered accumulation of ROS, measured in a peroxidase assay as emitted photons. Accumulation of ROS is shown over time (C) or as total count (D).