PregS (25 µM) increases AMPA-mEPSC frequency by elevating [Ca2+]i possibly via modulation of TRP channels. (A) Sample traces illustrating the effect of PregS on AMPA-mEPSC frequency in PCs from P4-5 rats in the presence of BAPTA-AM, Cd2+ and La3+, calibration: 50 pA and 0.2 s. (B) Summary graphs of the percent frequency (left panel) and the raw frequency (right panel) changes induced by PregS in the presence of these agents. For both, percent and raw frequency increases, one-way ANOVA followed by Dunn's multiple comparison test showed a significant difference between controls (n=11), BAPTA-AM (n=9; * p < 0.05) and La3+ (n=11; *** p < 0.001). The effect of PregS on AMPA-mEPSC frequency from Fig 1 is shown again for comparison.