Table 1.

Transcription and promoter methylation analyses of CLIC2 and PIN4 in PBC twin pairs

CLIC2			PIN4
Relative transcript levela	Overall % Methylationb	Relative transcript levela	Overall % Methylation Allele 1c	PIN4 genotyped	Sample ID	Age (years)	Status	Twin status
5.80	62%	16.11	36%	1, 1	1	60	Healthy	Discordant
3.46	61%	21.11	56%	1, 1	2	60	PBC	Discordant
5.50	48%	22.47	n/a	1, 1	52	68	Healthy	Discordant
2.73	60%	8.11	49%	1, 1	9	68	PBC	Discordant
3.92	52%	39.4	50%	1, 2	54	63	Healthy	Discordant
1.88	44%	17.27	34%	1, 2	55	63	PBC	Discordant
1.00	63%	10.06	n/a	1, 1	18	64	Healthy	Discordant
2.11	61%	<LOD	n/a	1, 1	19	64	PBC	Discordant
6.73	55%	7.21	20%	1, 1	24	58	PBC	Concordant
8.17	67%	5.86	39%	1, 1	57	58	PBC	Concordant

^alineared values, weakest normalized calibrator

^bnumber of methylated CpG sites out of total number of CpG sites assayed

^cnumber of methylated CpG sies out of 11 CpG sites assayed per clone in allele 1 dallele 1 has 11 CpG sites in promoter region, allele 2 has 13 CpG sites.