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. 2011 Jan 10;108(4):1633–1638. doi: 10.1073/pnas.1013262108

Fig. 3.

Fig. 3.

LLO oligomerization and hemolytic activity is enhanced in vitro by high KCl concentration. (A) SDS/agarose gel electrophoresis analysis of lysates followed by immunoblotting using an anti-LLO antibody. (B and C) Hemolytic activity of rLLO lysates at pH 5.5 without (closed triangles) or with (open symbols) increasing KCl. BL21(DE3) lysate (triangles) and Triton X-100 (diamonds) represent negative and positive controls, respectively. (D) Hemolytic activity of rLLO lysates at pH 5.5 in HAB (closed triangles) or HAB with 428 mM KCl (open squares), NaCl (open diamonds), or KOAc (open circles). (E) Hemolytic activity of BL21(DE3) lysates expressing rLLO (triangles) or rL461T (squares) with (open symbols) or without (closed symbols) 428 mM KCl on SRBC at pH 5.5. Results shown in all panels represent at least three independent experiments.