Fig. 5.
SPI-1–dependent translocation of purified YopH1–99-Bla into HeLa cells infected with coated S. typhimurium strains. (A) Translocation of purified YopH1–99-Bla into HeLa cells infected with coated S. typhimurium strains; LT2 (WT + YopH1–99-Bla) and a polar invA insertional mutant (invA– + YopH1–99-Bla). Translocation is seen as blue fluorescence. (B) Translocation of purified YopH1–99-Bla into HeLa cells by coated S. typhimurium strains. Data are presented as mean ± SEM of six separate experiments corresponding to more than 1,000 cells. (C) Coating of externally added, purified YopH1–99-Bla on S. typhimurium strains presented as number of molecules per cell (mean ± SEM of four independent experiments).