Fig. 3.

TD immune responses. (A and B) Functional CSR. WT and KO mice (n = 6 per group) were i.p. injected with 100 μg NP₍₁₅₎-CG and serum levels of NP₍₁₅₎-CG–specific IgM (A) and IgG (B) were determined by ELISA on days 7, 14, or 21 after immunization. Day 0, preimmunization. Data are Ig titers of individual mice. Horizontal bars represent mean values (*P < 0.05; **P < 0.005; ns, not significant). (C) Increased spontaneous GC formation. WT and KO mice (n = 3 per group) were i.p. injected with 100 μg NP₍₁₅₎-CG. Before immunization (control), or on day 14 after immunization, spleen sections were stained with anti-B220 (brown) plus PNA (blue) to detect GCs.