Table 1.

Cellularity of B-cell subsets in WT and 14-3-3σ–deficient (i.e., KO) mice

	Cellularity
Subset BM	Total	IgM−	IgM+	Recirculating
WT	8.6 ± 1.1	4.3 ± 0.7	2.1 ± 0.4	1.92 ± 0.01
KO	8.5 ± 1.3	4.3 ± 0.9	3.3 ± 0.3	0.39 ± 0.06
Spleen*	Total	imm†	FO	MZ
WT	14.5 ± 0.3	1.6 ± 0.07	11.1 ± 0.5	0.8 ± 0.14
KO	4.8 ± 2.1	1.18 ± 0.6	3.0 ± 1.2	0.01 ± 0.005

Total B220⁺ B cells (total) from BM were immunostained and fractionated by FACS into subsets: B220⁺IgM⁻ B-cell progenitors (IgM⁻), B220^lowIgM⁺ immature B cells (IgM⁺), and B220^highIgM⁺ recirculating B cells. Values shown are absolute cell numbers (×10⁻⁶).

*Total splenic B220⁺ B cells (total) were immunostained and fractionated by FACS into subsets: CD21⁻CD23⁻ immature B cells (imm), CD21^lowCD23^high FO B cells (FO), and CD21^highCD23^low MZ B cells (MZ).

^†imm, immature.