Fig. 3.

Nucleosome cooccupancy of old and new H3. Samples from the experiments described in Figs. 1C and 2 were subjected to two rounds of immunoprecipitation using VSVG and HA antibodies. The graphs show the average results of sequential ChIP analysis, relative to the values of the single-induction sample E, regarded as experimental background. H3 cooccupancy was analyzed at a telomeric locus (TELVI-R) and six different genes, with the positions relative to the translation start site indicated.