Skip to main content
. 2011 Jan 4;108(4):1681–1686. doi: 10.1073/pnas.1016424108

Fig. 5.

Fig. 5.

HSF1 regulates the expression of St8siaII and St8siaIV. (A) Western blotting showing the expression levels of HSF1 and PSA-NCAM proteins in the hippocampus and the medial prefrontal cortex of HSF1+/+ mice at different developmental stages. (B) Real-time PCR analysis showing the expression levels of St8siaII and St8siaIV mRNA in the hippocampus and the medial prefrontal cortex of HSF1+/+ mice at differential developmental stages. PND, postnatal day. (C and D) Real-time PCR analysis showing the expression levels of (C) St8siaII and (D) St8siaIV mRNA in the hippocampus and the medial prefrontal cortex of HSF1+/+, HSF1+/−, and HSF1−/− mice on postnatal days 2 (P2) and 56 (P56) (n = 6 for each group). (E) ChIP assay with HSF1 antibody (α-HSF1) or preimmune serum (PI) as well as with input DNA shows the recruitment of HSF1 to the St8siaII and St8siaIV promoters in the neonatal hippocampus of mice (postnatal day 2). The transcription start site is denoted as +1. Underscoring indicates the positions of the PCR primers (P1–P6) used in the ChIP assay. (F and G) Overexpression of caHSF1 in the neonatal hippocampus of HSF1−/− mice restored the expression of (F) St8siaII and St8siaIV mRNA and (G) PSA-NCAMs (n = 8 for each group). *P < 0.05.