Fig. 6.

Establishment of silencing over time visualized continuously by quantitative microscopy. DOT1 (JRY9101) or dot1Δ (JRY9104) cells were grown in 5 mM nicotinamide to derepress the hml::GFP::PEST::NLS reporter gene, and silencing establishment was then initiated by washing nicotinamide out of the media. As a control, DOT1 cells were maintained in 5 mM nicotinamide media for the duration of the time course. The fluorescence intensity of each cell is illustrated as a line plot. Each graph represents ∼10–20 starting cells and all their subsequent descendants.