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Potency of thiaisoleucine and mupirocin depends on exogenous isoleucine concentration. Parasite growth in the presence of 20 μM, 80 μM, or 200 μM isoleucine and either thiaisoleucine (A) or mupirocin (B). Growth of triplicate cultures at increasing concentration of drug was measured by flow cytometry. Data points are shown as mean ± range. Curves show least-square fits of data points. IC50 values and SEs are provided in SI Appendix, Table S1.
