Fig. 1.

Isolation of M. kandleri RNA splicing ligase. (A) Purification scheme. RNA ligase activity was purified from soluble protein extract (S20) by three consecutive steps. (B) Purified fractions from the Mono Q column. Proteins bound to the Mono Q HR5/5 column were eluted in a 15-mL linear gradient from 0–500 mM NaCl and collected in 20 fractions. Aliquots of the Mono Q elution fractions in the range from 400–500 mM NaCl were analyzed on a 4–20% gradient polyacrylamide/0.1% SDS gel and stained with Coomassie colloidal blue (Pierce Imperial Stain). The arrow indicates the putative RNA ligase protein.