Fig. 4.

Overexpression of CDCP1 enhances Src family kinase activity. (A) Western blotting using antibodies specific for activated forms of SFKs or Akt shows that CDCP1 overexpression in A375 cells enhances activation of SFKs and Akt. A point mutation at Y734F in CDCP1 abolished this enhancement, whereas mutations at Y743F and Y806F had no effect. Relative signal intensities (RSI) for pSFK and pAKT are indicated. (B) Treatment with Src-family kinase inhibitor, Dasatinib, abolished scattered growth of A375-CDCP1 cells in 3D Matrigel and was imaged 6 d later. (Scale bar: 30 μm.)