Fig. 4.

RNAi of WNK1 resulted in a defect in abscission. Immunostaining of HeLa cells 72 h after transfection with scrambled (A) or WNK1 siRNA (B) oligonucleotides. WNK1 (red), tubulin (green), and DAPI (blue channel). In A and B, the bridge between two daughter cells is overlaid with a bar indicating length. (Scale bar: 15 μm.) (C) Average length of bridges in scrambled control and WNK1 knockdown cells is plotted. Sample images were taken from four independent experiments (scrambled control, n = 80; WNK1 knockdown, n = 90). Statistical analysis was performed by an unpaired t test. Individual frames from time-lapse microscopy of scrambled control (D) and WNK1 knockdown (E) cells. Regions between separating cells are indicated by yellow arrowheads. Perimeters of separated daughter cells in the scrambled control are indicated by yellow lines in D.