Depletion of endogenous OSR1 does not cause defects in mitotic spindles. Immunostaining of HeLa cells 48 h after transfection with scrambled (A–C) or OSR1 siRNA (D–I) oligonucleotides. OSR1 (A and D; red) or WNK1 (G; red), tubulin (B, E, and H; green), and DAPI (C, F, and I; blue). (Scale bar: 5 μm.)