Fig. 4.

Suppression of HA-specific T cells by J immunization in vitro and in vivo. a Ag-dependent suppression of clone 4 T cells in vitro. Naive, CFSE-labeled CD8⁺CD45.1⁺ clone 4 T cells were incubated with CD4⁻ or CD4⁺ splenocytes from mice immunized with HA or J peptide in the presence of 10 μM peptide and analyzed after 3 days. b Ag-dependent suppression of clone 4 T cells in vivo. CD45.1/CD45.2 heterozygous B10.D2 mice with and without prior depletion of CD25⁺ cells were immunized with DC loaded with equal amounts of HA and either CDR3 or J peptide. 3 days later, CFSE-labeled, CD45.1^+/+CD8⁺ clone 4 cells were injected i.v. 1 week after immunization, CD8⁺CD45.2⁻ and HA peptide/H-2K^d multimer-positive splenocytes were measured. c Effects of co-immunization with HA and J peptide on the induction of HA-specific T cells. BALB/c mice were immunized with DC loaded with equal concentrations of HA and J peptide (co-immunized) or immunized separately into contralateral hind paws with DC loaded only with HA or J peptide. HA-specific T cells were measured after 1 week